Day 1 :
Keynote Forum
Safaa Aldahlawi
Keynote: Gene Expression Profiling on CML Patients with Philadelphia Translocation
Time : 10:00-10:20
Biography:
Safaa AlDahlawi is Molecular Lab Specialist- Center of Excellence in Genomic Medicine and Hematology Msc -King Abdulaziz University Saudi Arabia.
Abstract:
Tyrosine Kinase Inhibitors TKIs and other targeted medicines are crucial in the management of CML patients' treatment. However, some patients do continue to experience less favorable and exhibit resistance to therapy. Therefore, the aim of our study is to conduct whole transcriptome sequencing to assess the differential gene expression profile in CML cases according to the patient’s response to TKI-based therapy.Method: Ten samples of human blood taken from CML patients at two different phases (diagnosis stage and relapse stage). All samples were subjected to RNA extraction, and ≤20 µg of RNA were used for NGS sequencing. The DESeq2 R software was used to do the data analysis. Result: A list of 499 genes was found to have significantly differential expression levels in the two study groups in CML cases. 122 genes were discovered to be upregulated in the analysis of gene expression, whereas 377 were found to be downregulated. All deregulated genes showed expression changes greater than |log2FoldChange|>0. There was a highly significant deregulation of NTRK2 (>+5 fold change) and KRT17 (< -5 fold change) expression compared to other genes. The majority of the expressed genes are involved the cell cycle, PI3K-AKT signaling pathway, cellular senescence, oxidative phosphorylation, microRNA in cancer, FOXO, and P53 signaling pathways, as well as other biological processes
Keynote Forum
Mohamed Abdel-haleem Hegazy El-kady
Keynote: Correlation between Polyaromatic Hydrocarbons (PAHs) Discharges at El-Manzala Wetland with the Relative Gene Expression of CYP19 Gene of the Nile Tilapia (Oreochromis niloticus)
Biography:
Dr. Mohamed El-kady has completed his PhD at the age of 39 years from laboratory of marine biotechnology, Kagoshima University, Japan and postdoctoral studies from the same University. He is the head of laboratory of genetics and genetic engeineering, Aquaculture Division, Institute of Oceanography and Fisheries, Egypt. He has published more than 20 papers in reputed journals related with cytochrome P450 genes; strcture and differntial expressions in various organs of fish
Abstract:
El-Manzala Wetland is known to be of the most significant sources of inexpensive different fish species for human consumption in Egypt. The Lake receives pollution from various sources; domestic wastewater, industrial wastewater, and agricultural drainage directly without any treatments, which leads to many environmental disorders. The following research was conducted for lake water quality evaluation and how it impacts the expression of the aromatase (CYP19a&b) genes in Oreochromis niloticus tissues at different locations of El-Manzala Lake. Sampling was done during the summer of 2018 when water and fish samples were collected from five different locations of El-Manzala Lake. It was found that the studied locations recorded hazardous concentrations of polyaromatic hydrocarbons (PAHs) that may cause significant defects to aquatic organisms except in the reference location and affect the expression of CYP19a&b genes. The relative gene expressions of CYP19a in the ovary and the testis were measured and recorded in positive ranges but revealed the controversial expression pattern of CYP19b gene in the brain tissues. Our results revealed that PAHs discharges at El-Manzala Lake are correlated with the gene expression of CYP19 genes. The current findings suggested a correlation between biomarkers response and wastewater exposure as stressors. Besides, the assessed multiple general health indicators give prospective insights for Nile tilapia adaptive pattern and monitoring of pollution of freshwater ecosystems